CFP-Rab9 does not colocalize with YFP-TIP47. (A–H) HeLa cells were transiently transfected with YFP-TIP47 (A and E, green), CFP-Rab9 (B and F, green), CFP-Rab9, and YFP-TIP47 (C and G; green, CFP; red, YFP) or with CFP-ADRP and YFP-TIP47 (D and H; green, CFP; red, YFP). Cells depicted in the bottom panels were fixed after oleic acid (OA) feeding. A neutral lipid stain is shown in red (E and F) or blue (G and H). Brightness and contrast of TIP47 staining in A and E were enhanced to visualize the otherwise faint cytoplasmic staining. Colocalization is indicated in yellow. Bar, 15 µm.