Figure 2. Loss of Mvb12 reveals sorting defect of vps23ΔUb and vps36ΔUb mutants. (A) GFP-Cps1 localization was assessed in vps23ΔUb1 (PLY3528) and vps36ΔUb (PLY3395) cells. GFP fluorescence images along with matching DIC images are shown. (B) Sorting of GFP-Cps1, Ste3-GFP–Ub, and Ste3-GFP in vps23ΔUb1 vps36ΔUb (PLY3522), mvb12Δ-null (JPY21), vps23ΔUb1 vps36ΔUb mvb12Δ (PLY3623), and vps23ΔUb1 mvb12Δ (PLY3624) cells. (C) GFP-Cps1 from wild-type (WT) and vps23ΔUb1 vps36ΔUb mvb12Δ cells was immunoprecipitated with anti-GFP antibodies and immunoblotted with anti-Ub and anti-GFP antibodies. Black line indicates that intervening lanes have been spliced out. IP, immunoprecipitation. Bars, 5 µm.
Figure 2.

Loss of Mvb12 reveals sorting defect of vps23ΔUb and vps36ΔUb mutants. (A) GFP-Cps1 localization was assessed in vps23ΔUb1 (PLY3528) and vps36ΔUb (PLY3395) cells. GFP fluorescence images along with matching DIC images are shown. (B) Sorting of GFP-Cps1, Ste3-GFP–Ub, and Ste3-GFP in vps23ΔUb1 vps36ΔUb (PLY3522), mvb12Δ-null (JPY21), vps23ΔUb1 vps36ΔUb mvb12Δ (PLY3623), and vps23ΔUb1 mvb12Δ (PLY3624) cells. (C) GFP-Cps1 from wild-type (WT) and vps23ΔUb1 vps36ΔUb mvb12Δ cells was immunoprecipitated with anti-GFP antibodies and immunoblotted with anti-Ub and anti-GFP antibodies. Black line indicates that intervening lanes have been spliced out. IP, immunoprecipitation. Bars, 5 µm.

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