Collagen IV staining reveals disorganized collagen deposition and more scattered infiltrate in SPARC^−/− mice. SPARC^+/+ (top row) and SPARC^−/− (bottom row) mice were injected in the skin with 10⁷ CFU of attenuated S. typhimurium SL3261 AT. Immunohistochemical staining with anti–collagen IV (brown) and counterstaining of nuclei with hematoxylin (blue) is shown on sections from mice killed 5 d after infection. Numbers show the original magnification of the sections. Boxes represent the magnified areas in the adjacent right images. Bars: (left) 50 μm; (middle) 25 μm; (right) 5 μm.