Phenotypic analysis of vaccine-induced CD4 and CD8 T cells. Blood mononuclear cells obtained from volunteer ES26, that was vaccinated with DNA C plus NYVAC C, were stimulated with Env-derived peptide pools or with CMV/EBV-derived peptides for 16 h and stained with IL-2, IFN-γ, CD4, CD8, CD45RA, and CCR7 antibodies. The blue dots indicate Env-specific (IL-2 plus IFN-γ) vaccine-induced CD4 T cells. The red dots indicate Env-specific (IL-2 plus IFN-γ) vaccine-induced CD8 T cells. The green dots indicate CMV/EBV-specific (IL-2 plus IFN-γ) vaccine-induced CD8 T cells.