Figure 4.
Vaccine-induced CD4 and CD8 T cell responses. (A) Flow cytometry profiles of vaccine-induced CD4 and CD8 T cell responses directed against Env in six out of the seven responders vaccinated with DNA C plus NYVAC C exhibiting CD4 and CD8 T cell responses. CD4 and CD8 T cell responses were defined using polychromatic flow cytometry. Blood mononuclear cells were stimulated with the relevant peptide pools and stained with IFN-γ, CD4, and CD8 antibodies. (B) Correlation of the IFN-γ–secreting cells measured by both flow cytometry and ELISPOT assay. This comparison was performed in the 17 volunteers with vaccine-induced T cell responses in the range of 100 SFU/106 cells or above.
