Magnitude of the vaccine-induced T cell responses. (A) Individual patterns of the T cell responses as measured by the frequencies of IFN-γ–secreting cells against different peptide pools encompassing the Env, Gag, Pol, and Nef proteins in an ELISPOT assay are shown for all responders in both study groups. Each bar corresponds to the reactivity against a different peptide pool, and positive responses (against Gag, Pol, and Nef) are indicated by a star. The Env-specific responses correspond to the sum of the mean of the responses induced after stimulation with the two Env peptide pools. (B) Median magnitude of vaccine-induced T cell responses against Env at different time points across the duration of the study. (C) Median magnitude of Gag-, Pol-, and Nef-specific vaccine-induced T cell responses at weeks 26 and 28. (D) Cumulative distribution of the sum of SFU/106 cells of positive responses at week 26 or 28 by randomization group. For each participant, the week of maximum T cell response (sum SFU) was chosen. Each step corresponds to the maximal IFN-γ ELISPOT value for each participant.
