RNA- and codon-optimized GPN and Env gene vector inserts. (A and B) Schematic representation of EV02 study design. ΔMyr, myristoylation-deficient; FS (-1), placing Gag and PolNef in one reading frame by removing the natural frameshift; ΔPR, protease-inactivated; RT-N, RT-C NH2 terminal and C-terminal part of the HIV reverse transcription; SC-Nef, scrambled Nef; RT-AS, active site of RT; SP, signal peptide.