Figure 1. GATA-1 functions independent of FOG cofactors in mast cell development. (A) Schematic diagram showing hierarchical relationships of the hematopoietic progenitor populations examined in B. (B) RT-PCR analysis of GATA-1, GATA-2, and FOG-1 expression in sorted progenitor cell populations, day 3 MCPs (d3 MCP), and peritoneal mast cells. FcεRI, MMCP-1, and MMCP-5 are included as mast cell–specific marker genes. HPRT serves as the housekeeping gene control. White lines indicate that intervening lanes have been spliced out. (C) RT-PCR analysis of FOG-2 expression in BMMCs (from 6-wk-old cultures) or whole mouse heart tissue. MMCP-2, MMCP-4, and MC-CPA are included as mast cell–specific markers. (D) May-Grunwald-Giemsa stains and FACS analysis for FcεRI and c-kit expression of YSMCs from wild-type, GATA-1−, or GATA-1V205G(KI) male embryos. Bars, 10 μm.
Figure 1.

GATA-1 functions independent of FOG cofactors in mast cell development. (A) Schematic diagram showing hierarchical relationships of the hematopoietic progenitor populations examined in B. (B) RT-PCR analysis of GATA-1, GATA-2, and FOG-1 expression in sorted progenitor cell populations, day 3 MCPs (d3 MCP), and peritoneal mast cells. FcεRI, MMCP-1, and MMCP-5 are included as mast cell–specific marker genes. HPRT serves as the housekeeping gene control. White lines indicate that intervening lanes have been spliced out. (C) RT-PCR analysis of FOG-2 expression in BMMCs (from 6-wk-old cultures) or whole mouse heart tissue. MMCP-2, MMCP-4, and MC-CPA are included as mast cell–specific markers. (D) May-Grunwald-Giemsa stains and FACS analysis for FcεRI and c-kit expression of YSMCs from wild-type, GATA-1−, or GATA-1V205G(KI) male embryos. Bars, 10 μm.

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