Figure 5. Bipolar spindle formation precedes centrosome detachment in the absence of mitotic NuMA function. (A and B) Selected images from videos of primary embryo fibroblasts transduced with retrovirus encoding tubulin-YFP, either heterozygous (A; Video 1, available) or homozygous (B; Video 2) for NuMAΔ22, undergoing the first mitosis after 4-OHT treatment and release from growth arrest. Arrows indicate centrosomes, and each time point shows a maximum intensity projection of five confocal fluorescence z sections acquired in 2-µm intervals.
Figure 5.

Bipolar spindle formation precedes centrosome detachment in the absence of mitotic NuMA function. (A and B) Selected images from videos of primary embryo fibroblasts transduced with retrovirus encoding tubulin-YFP, either heterozygous (A; Video 1, available) or homozygous (B; Video 2) for NuMAΔ22, undergoing the first mitosis after 4-OHT treatment and release from growth arrest. Arrows indicate centrosomes, and each time point shows a maximum intensity projection of five confocal fluorescence z sections acquired in 2-µm intervals.

This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal