Figure 1.
Figure 1. In vivo limiting dilution analysis to determine functional precursor frequencies of NP-, PA-, and PB1-specific CD8 T cells. The indicated numbers of CD8 T cells isolated from young naive C57BL/6 donor mice were adoptively transferred into young TCRβ/δ−/− recipient mice 1 d before infection with influenza virus. Lung tissue was harvested from individual recipient mice 14 d after infection. Each FACS dot plot shows the frequency of donor lymphocytes present within the lung tissue of individual recipient mice that stained positive for both CD8 and either influenza NP (NP366-374/Db), PA (PA224-233/Db), or PB1 (PB1703-711/Kb) MHC class I tetramer. The data are representative examples of analysis of 3–4 mice per dilution, shown in Fig. 2.

In vivo limiting dilution analysis to determine functional precursor frequencies of NP-, PA-, and PB1-specific CD8 T cells. The indicated numbers of CD8 T cells isolated from young naive C57BL/6 donor mice were adoptively transferred into young TCRβ/δ−/− recipient mice 1 d before infection with influenza virus. Lung tissue was harvested from individual recipient mice 14 d after infection. Each FACS dot plot shows the frequency of donor lymphocytes present within the lung tissue of individual recipient mice that stained positive for both CD8 and either influenza NP (NP366-374/Db), PA (PA224-233/Db), or PB1 (PB1703-711/Kb) MHC class I tetramer. The data are representative examples of analysis of 3–4 mice per dilution, shown in Fig. 2.

This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal