Figure 2. ITC analysis of binding of WT and mutant Munc18-1 to syntaxin-1. (A–D) Illustrative examples of the ITC data obtained for binding of WT Munc18-1 (A) and E59K (B), E66A (C), and K63E (D) Munc18-1 mutants to syntaxin-1(2–243) are shown. A polynomial baseline correction was applied to remove a slight drift in the initial points of each titration before fitting the data to a single-site binding model. This correction did not substantially alter the Kd values obtained.
Figure 2.

ITC analysis of binding of WT and mutant Munc18-1 to syntaxin-1. (A–D) Illustrative examples of the ITC data obtained for binding of WT Munc18-1 (A) and E59K (B), E66A (C), and K63E (D) Munc18-1 mutants to syntaxin-1(2–243) are shown. A polynomial baseline correction was applied to remove a slight drift in the initial points of each titration before fitting the data to a single-site binding model. This correction did not substantially alter the Kd values obtained.

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