Figure 5. Ribbon determinants partition together with the spindle. Rescue of the ribbon by incorporation of spindle materials. Cells induced to divide asymmetrically at 37°C (A–D and Video 1, available) or 32°C (E–H and Video 2) were followed by video microscopy and stained for GFP (B and F), tubulin (C and G), and DNA (D and H). At 37°C, the spindle was located distal to the division plane (A). Microtubules (MT) were not partitioned into the cytoplasts (C, asterisk), and the Golgi was scattered (B, asterisk). At 32°C, the spindle was positioned close to the cleavage furrow (E). Spindle microtubules were incorporated into the cytoplasts (G, asterisk), and a Golgi ribbon reformed (F, asterisk). Karyoplasts are marked by arrows, and asterisks indicate cytoplasts. Bar, 10 µm.
Figure 5.

Ribbon determinants partition together with the spindle. Rescue of the ribbon by incorporation of spindle materials. Cells induced to divide asymmetrically at 37°C (A–D and Video 1, available) or 32°C (E–H and Video 2) were followed by video microscopy and stained for GFP (B and F), tubulin (C and G), and DNA (D and H). At 37°C, the spindle was located distal to the division plane (A). Microtubules (MT) were not partitioned into the cytoplasts (C, asterisk), and the Golgi was scattered (B, asterisk). At 32°C, the spindle was positioned close to the cleavage furrow (E). Spindle microtubules were incorporated into the cytoplasts (G, asterisk), and a Golgi ribbon reformed (F, asterisk). Karyoplasts are marked by arrows, and asterisks indicate cytoplasts. Bar, 10 µm.

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