Figure 4. Fus3p activity is required to... | Rockefeller University Press

Figure 4.

$Figure 4. Fus3p activity is required to maintain Fus2p in the cytoplasm. (A) FUS3 (MY10011) or FUS3-Q93G (MY10012) strains were pregrown in selective medium and induced with α factor for 90 min. Cells were placed on an agarose slide containing α factor with or without 1-NA-PP1, a selective inhibitor of Fus3p-Q93G (Matheos et al., 2004), and examined microscopically. (B) The fus3-Q93G strain (MY10012) was grown as in A and placed in a microscope flow cell chamber. Inhibitor was added at t = 0, and images were collected at 1-min intervals. (C) GFP fluorescence in the nucleus and at the shmoo tip was measured in 11 cells treated as in B and expressed as a fraction of t = 20 min (nucleus) or t = 0 (tip). Closed symbols, relative shmoo tip fluorescence; open symbols, relative nuclear fluorescence (see Materials and methods). Error bars represent ± SEM. Bars, 1 µm.$

Fus3p activity is required to maintain Fus2p in the cytoplasm. (A) FUS3 (MY10011) or FUS3-Q93G (MY10012) strains were pregrown in selective medium and induced with α factor for 90 min. Cells were placed on an agarose slide containing α factor with or without 1-NA-PP1, a selective inhibitor of Fus3p-Q93G (Matheos et al., 2004), and examined microscopically. (B) The fus3-Q93G strain (MY10012) was grown as in A and placed in a microscope flow cell chamber. Inhibitor was added at t = 0, and images were collected at 1-min intervals. (C) GFP fluorescence in the nucleus and at the shmoo tip was measured in 11 cells treated as in B and expressed as a fraction of t = 20 min (nucleus) or t = 0 (tip). Closed symbols, relative shmoo tip fluorescence; open symbols, relative nuclear fluorescence (see Materials and methods). Error bars represent ± SEM. Bars, 1 µm.

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