Fus2p transiently localizes to the cytoplasm in far1 mutants. (A) Fus2p-GFP localization was examined in a far1 mutant (MY10320) as described in Fig. 1 B. In representative images, Fus2p-GFP transiently localized to the cytoplasm in both cells (top), in the bud alone (middle), or in neither (bottom). (B) In medium- to large-budded far1 cln2 mutant cells (MY10321), Fus2p-GFP exited after cytokinesis, and cells arrested in G1 (top). In small-budded cells, Fus2p-GFP was present in the cytoplasm. Similar results were obtained when Fus2p-GFP was expressed from the GAL1 promoter. Bars, 1 µm.