Figure 2. Cell cycle reentry requires RBP-Jκ. (A) Examples of RBP-JκFlox/Flox NMVCs infected with Ad-Cre-EGFP to remove RBP-Jκ, then reinfected 12 h later with Ad-N2ICD, as indicated, cultured 48 h, and stained with MF20 (red, Alexa 594), Ki67 (far red, Cy5, shown as green), and DAPI (blue). Arrows indicate Ki67+ cells. Bar, 10 μm. (B) The percentage of Ki67+ cardiomyocytes (MF20+) in each condition. Only Cre-expressing cells (GFP+, green) were counted in cultures infected with Ad-Cre-EGFP. N2ICD induced KI67 (P = 0.05) but the N2ICD effect following Cre was not significantly different from that of Cre alone (ns). Error bars indicate ±SD across experimental replicates.
Figure 2.

Cell cycle reentry requires RBP-Jκ. (A) Examples of RBP-JκFlox/Flox NMVCs infected with Ad-Cre-EGFP to remove RBP-Jκ, then reinfected 12 h later with Ad-N2ICD, as indicated, cultured 48 h, and stained with MF20 (red, Alexa 594), Ki67 (far red, Cy5, shown as green), and DAPI (blue). Arrows indicate Ki67+ cells. Bar, 10 μm. (B) The percentage of Ki67+ cardiomyocytes (MF20+) in each condition. Only Cre-expressing cells (GFP+, green) were counted in cultures infected with Ad-Cre-EGFP. N2ICD induced KI67 (P = 0.05) but the N2ICD effect following Cre was not significantly different from that of Cre alone (ns). Error bars indicate ±SD across experimental replicates.

This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal