Recruitment of pRLC to the cell cortex requires Rho activity. Single medial confocal sections of fixed and stained D. excentricus embryos at first (A–F) and third (G) telophase. (A–C) Noninjected controls. (D–G) Zygotes injected with C3-transferase >15 min before NEB. (D–G) Coinjected fixable FITC-dextran labels the cytoplasm (to mark injected cells). A and D, B and E, and C and F are matched by mitotic phase. All cells were stained to show pRLC (lavender) and nuclei (green). Bar, 25 μm.