Equatorial recruitment of pRLC does not require F-actin. Surface views made from projecting 64 serial confocal sections (0.5 μm apart) of two sibling D. excentricus zygotes. Cells in B and D were cultured in 20 μM latrunculin (13°C); cells in A and C were cultured without latrunculin. Both cells were fixed during telophase. A and B show F-actin (stained with Bodipy FL phallacidin); C and D show pRLC. Identical preparation protocols and microscope settings were used for cells in A and B, and C and D, respectively. Bar, 35 μm.