Figure 7. The effect of a CK2 inhibitor on the accumulation of sodium channels at the AIS of cultured hippocampal neurons. Cultured hippocampal neurons were treated with either DMAT (50 μM) or with DMSO (control cells) either from 2 to 4 DIV (A and B) or from 9 to 10 DIV (C and D). They were subsequently immunostained for Nav1 channels (green), ankyrin G (red), and MAP2 (blue). (B and D) Quantification of Nav1 and ankyrin G staining intensity. Fluorescence intensity was normalized by taking as 100% the staining intensity measured in control cells. The results are from three independent experiments, the number of quantified AIS ranged from 70 to 230. Error bars indicate mean ± SEM. t test: ***, P < 0.0001. Bars, 10 μm.
Figure 7.

The effect of a CK2 inhibitor on the accumulation of sodium channels at the AIS of cultured hippocampal neurons. Cultured hippocampal neurons were treated with either DMAT (50 μM) or with DMSO (control cells) either from 2 to 4 DIV (A and B) or from 9 to 10 DIV (C and D). They were subsequently immunostained for Nav1 channels (green), ankyrin G (red), and MAP2 (blue). (B and D) Quantification of Nav1 and ankyrin G staining intensity. Fluorescence intensity was normalized by taking as 100% the staining intensity measured in control cells. The results are from three independent experiments, the number of quantified AIS ranged from 70 to 230. Error bars indicate mean ± SEM. t test: ***, P < 0.0001. Bars, 10 μm.

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