Yeast temporal analysis reveals accumulation of Pab1 in P bodies before formation of P body–distinct stress granules. (A) Log-phase yRP840 strain, transformed with pRP1660, was glucose deprived for 5 min and followed over time. For technical reasons, identical cells at the 0-min time point are not shown; instead a typical image of this time point is shown. Orange arrowheads indicate initial accumulation of Pab1-GFP in preexisting P bodies. Purple arrowheads indicate appearance of P body–distinct stress granules. Images are typical of multiple independent experiments. Images were taken from Video 1 . (B) Zoom panels from the 19-min time point (white boxes). Arrowheads indicate common occurrence of faint P-body foci colocalizing with stress granules. Purple arrowhead indicates same foci in A, turquoise arrowheads indicate location of additional faint P bodies. Right: Dcp2-mCh image at 10x normal scaling intensity, indicating relative difference in Dcp2-mCh abundance between P bodies and stress granules. Asterisk indicates auto-fluorescent vacuolar signal.