Figure 4. Mutations of Cdk sites in SMRT disrupt Pin1 interaction. (A) Phosphospecific antibodies recognize WT but not mutant SMRT. HeLa cells were transfected with either WT HA-SMRT (1,178–1,823) or a 3× mutant (S1241A/T1445A/S1469A), and WCEs were prepared for immunoblotting with the indicated antibodies. (B) Mutation of Cdk sites in SMRT disrupts Pin1 interaction in vitro. HeLa cells were transfected with HA-SMRT WT (1,012–2,507) or the 3× mutant, and WCEs were subjected to pull downs with GST–Pin1 and immunoblotted with α-HA antibodies. (C) Mutation of Cdk sites stabilizes SMRT. HeLa cells were transfected with HA-SMRT (1,178–1,823) or the 3× mutant and treated with 100 μg/ml CHX for the indicated times. WCEs were immunoblotted with the indicated antibodies. Left, immunoblot; right, quantification of SMRT levels normalized to actin levels.
Figure 4.

Mutations of Cdk sites in SMRT disrupt Pin1 interaction. (A) Phosphospecific antibodies recognize WT but not mutant SMRT. HeLa cells were transfected with either WT HA-SMRT (1,178–1,823) or a 3× mutant (S1241A/T1445A/S1469A), and WCEs were prepared for immunoblotting with the indicated antibodies. (B) Mutation of Cdk sites in SMRT disrupts Pin1 interaction in vitro. HeLa cells were transfected with HA-SMRT WT (1,012–2,507) or the 3× mutant, and WCEs were subjected to pull downs with GST–Pin1 and immunoblotted with α-HA antibodies. (C) Mutation of Cdk sites stabilizes SMRT. HeLa cells were transfected with HA-SMRT (1,178–1,823) or the 3× mutant and treated with 100 μg/ml CHX for the indicated times. WCEs were immunoblotted with the indicated antibodies. Left, immunoblot; right, quantification of SMRT levels normalized to actin levels.

This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal