Figure 2.
![Figure 2. Phosphatidylinositol bisphosphate specificity for syt-regulated fusion. (A) Fusion assays were performed as described in Fig. 1 B but in the presence of the indicated [neomycin]. (B) Quantification of the data from A. (C and D) The same experiments were performed as in A and B but using fusion assays in which FL syt was replaced by 3 µM C2AB. (E) Tr containing three different phosphatidylinositol bisphosphates was mixed with Vr-syt vesicles, and fusion was monitored as in Fig. 1 B. (F and G) The extent (F) and initial rate (G) of regulated fusion were calculated and plotted. Data were obtained from three or more independent trials. All data shown are represented as mean ± SEM.](https://cdn.rupress.org/rup/content_public/journal/jcb/195/7/10.1083_jcb.201104079/9/jcb_201104079_fig2.jpeg?Expires=1771763794&Signature=lGxN9RynytA40hApJi82QDJxfi~S6bpoYOCy4IMyhpMw-aarRofRlAfixTmF5biBwyfH9R1hj1Sv~tFZroYrcfYrnQ9YPhhc3mq997ygqOPcr9Q5VWq3RxLnomMHb~YMSSsjkTQL-wPn~ZoEGkXnEuk0gCDljvfAoG2Jap80N4lQcpIpEEGHI633RkF5yxPqrYU7TZ-upIjG5jddllhvzP6dyPeP6gNaCXSA0jL8lgdlMNTBa-tSeGd4lm7OaZ4QmTOu1VTIHApPoJgXJdjBxSMuSiak1jAxySwVDCXfQKoyRjw7qyhDWQ7uYOtdjY4fKb6gkOFtQH-mb9Fh91roDw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Phosphatidylinositol bisphosphate specificity for syt-regulated fusion. (A) Fusion assays were performed as described in Fig. 1 B but in the presence of the indicated [neomycin]. (B) Quantification of the data from A. (C and D) The same experiments were performed as in A and B but using fusion assays in which FL syt was replaced by 3 µM C2AB. (E) Tr containing three different phosphatidylinositol bisphosphates was mixed with Vr-syt vesicles, and fusion was monitored as in Fig. 1 B. (F and G) The extent (F) and initial rate (G) of regulated fusion were calculated and plotted. Data were obtained from three or more independent trials. All data shown are represented as mean ± SEM.
