Figure 8. Localization of endogenous pri-miRNAs. (A) Pri-miRNAs expressed at low levels were detected using amplification and localize to one or two foci per cell. (B) Pri-miR-34a is not detected in untreated HeLa cells (a–d) but shows diffuse nucleoplasmic localization in HeLa cells after UV irradiation (e–h). Costaining for SC35 is shown in panels b and f. (C) BIC/pri-miR-155 localizes throughout the nucleoplasm in Raji (a–d) and B95-8 cells treated with PMA and ionomycin (P/I) for 24 h, as well as in nuclear foci in B95-8 cells (e–h), many of which overlap with SC35 (g, arrows). (D) Pri-miR-BHRF1-2∼3 shows diffuse nucleoplasmic and cytoplasmic distribution in Raji cells (a–d) but is concentrated in nuclear foci in B95-8 cells (e–h), some of which overlap with SC35 (g, arrows). (E) EBV BHRF1 DNA localizes diffusely in the nucleus and cytoplasm (due to viral release). Bars, 10 μm.
Figure 8.

Localization of endogenous pri-miRNAs. (A) Pri-miRNAs expressed at low levels were detected using amplification and localize to one or two foci per cell. (B) Pri-miR-34a is not detected in untreated HeLa cells (a–d) but shows diffuse nucleoplasmic localization in HeLa cells after UV irradiation (e–h). Costaining for SC35 is shown in panels b and f. (C) BIC/pri-miR-155 localizes throughout the nucleoplasm in Raji (a–d) and B95-8 cells treated with PMA and ionomycin (P/I) for 24 h, as well as in nuclear foci in B95-8 cells (e–h), many of which overlap with SC35 (g, arrows). (D) Pri-miR-BHRF1-2∼3 shows diffuse nucleoplasmic and cytoplasmic distribution in Raji cells (a–d) but is concentrated in nuclear foci in B95-8 cells (e–h), some of which overlap with SC35 (g, arrows). (E) EBV BHRF1 DNA localizes diffusely in the nucleus and cytoplasm (due to viral release). Bars, 10 μm.

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