![Figure 3. Pri-miRNAs lacking a CPA signal are retained in chromatin, whereas ENE-containing pri-miRNAs are released. (A) Schematic of primers used for PCR in B and C. (B and C) Nuclear location of pri-miRNAs. Cells transfected with constructs diagramed in Fig. 2 A for pri-let-7 (B) or pri-lin-4 (C) were fractionated into released, nucleoplasmic RNAs (supernatant [S]) and chromatin-associated RNAs (pellet [P]). Fractionated RNA was reverse transcribed and amplified by PCR. β-actin pre-mRNA and spliced mRNA were amplified as controls for fractionation efficiency.](https://cdn.rupress.org/rup/content_public/journal/jcb/182/1/10.1083_jcb.200803111/7/jcb_200803111_rgb_fig3.jpeg?Expires=1767685420&Signature=akfRRTY8FWqOOyHfP8ZeZ96sICnzgSZpMweu2CCrIdLQ3XyOlcCKqzXgjuhxiqa~FP8wX9ncJ9PBQjd0OSelvaHdKhqe~AWvjT0rqGCMsIk-nOuvLWoE~Mtpb8vbeQ7LJLhe8nzV2ceRwbYkVR~jH07q8ZuBwgoSSDeDIkWGMf0dRuMIwA7XTN6M5PvzoGaNGI0EeFftxwuy04LX7r~1qXp1ULh9GwXaPMgn7dlgaHY3I3s~RZZPhlaXUCEBhSrSpd-J-Wo8BXfrt6d5Nk2qO9Bx9RK-G5dObylegswFTKuTsJQcxnMc-o0V3HVdlUa~SGF3m-WCL8YVPiHGgvBlZw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Figure 3.
Pri-miRNAs lacking a CPA signal are retained in chromatin, whereas ENE-containing pri-miRNAs are released. (A) Schematic of primers used for PCR in B and C. (B and C) Nuclear location of pri-miRNAs. Cells transfected with constructs diagramed in Fig. 2 A for pri-let-7 (B) or pri-lin-4 (C) were fractionated into released, nucleoplasmic RNAs (supernatant [S]) and chromatin-associated RNAs (pellet [P]). Fractionated RNA was reverse transcribed and amplified by PCR. β-actin pre-mRNA and spliced mRNA were amplified as controls for fractionation efficiency.
