GAPDH and NDPK-B aggregates are present in nuclei-enriched fractions of human alcoholic cirrhosis livers. (A) Cytoplasmic and nuclei-enriched fractions from two different normal human livers (NL) and liver explants of eight patients with alcoholic cirrhosis were analyzed by SDS-PAGE followed by blotting for GAPDH (reducing [R] or nonreducing [NR] conditions). Lamin B1 and β-tubulin were used as loading controls for the nuclear and cytoplasmic fractions, respectively. GAPDH aggregates (denoted by asterisks) are present in the diseased but not the normal human livers. (B) Analysis of NDPK-B expression in total liver lysates (under reducing conditions) from the same samples as in A. Higher molecular weight NDPK-B–containing complexes are present in the diseased but not the normal human livers.