Figure 6. Presence of... | Rockefeller University Press

Figure 6.

$Figure 6. Presence of detergent-insoluble and nuclear GAPDH in C57BL hepatocytes. (A) Hepatocytes from C57BL and C3H mice were cultured in presence of the indicated concentrations of DDC or 0.1% DMSO vehicle (0) or in the absence of DDC/DMSO (Control). Detergent-insoluble equal fractions were prepared and then blotted with antibodies to actin (loading control) and GAPDH. (B) Immunofluorescence-based localization of GAPDH in vehicle (DMSO; panels a–d) or DDC-treated hepatocytes (panels e–h) that were also double stained with DAPI (nuclear staining). Note that after DDC treatment, GAPDH translocates to the nucleus in C3H hepatocytes (arrows in panels e and f) and appears to aggregate in the perinuclear region of C57BL hepatocytes (arrows in panels g and h). Bar, 20 µm.$

Presence of detergent-insoluble and nuclear GAPDH in C57BL hepatocytes. (A) Hepatocytes from C57BL and C3H mice were cultured in presence of the indicated concentrations of DDC or 0.1% DMSO vehicle (0) or in the absence of DDC/DMSO (Control). Detergent-insoluble equal fractions were prepared and then blotted with antibodies to actin (loading control) and GAPDH. (B) Immunofluorescence-based localization of GAPDH in vehicle (DMSO; panels a–d) or DDC-treated hepatocytes (panels e–h) that were also double stained with DAPI (nuclear staining). Note that after DDC treatment, GAPDH translocates to the nucleus in C3H hepatocytes (arrows in panels e and f) and appears to aggregate in the perinuclear region of C57BL hepatocytes (arrows in panels g and h). Bar, 20 µm.