Hepatic differences in the expression, activity, and distribution of the cytoplasmic enzyme NDPK. (A) Relative expression level of NDPK-B in extrahepatic organs from C3H and C57BL mice. The actin blot serves as a loading control. (B) Total NDPK activity in C3H and C57BL mouse livers under basal conditions and after DDC treatment (top) and in untreated isolated hepatocytes (bottom). **, P < 0.01 using an unpaired t test; ***, P < 0.001 using a two-way analysis of variance. Each tested group included three to four mice, and samples were analyzed in triplicates. Results are represented as the mean and the SD. (C) Primary cultured hepatocytes from C3H (panels a–c) and C57BL (panels d–f) mice were triple immunostained for NDPK-B and K8 and were then mounted in the presence of DAPI as described in Materials and methods. Bar, 10 µm.