Strain-specific and DDC-induced changes in the expression of regulators of protein processing, energy metabolism, and oxidative stress. (A–C, top panels) Equal amounts of total liver protein from three untreated and four DDC-fed C3H or C57BL mice were resolved on SDS-PAGE gels and immunoblotted with antibodies to the indicated proteins. Blots separated by solid lines indicate nonconsecutive lanes of the same equally exposed membrane. The expression of some proteins (PDIA4, FAH, and ME1) was relatively unaffected across groups, and they serve as loading controls. (bottom panels) Relative band intensity values were plotted for each protein in the three different groups. ME1, malic enzyme 1; mono, monomer; oligo, oligomer. *, P < 0.05; **, P < 0.01; ***, P < 0.001 using a two-way analysis of variance. Each tested group included three to four mice. Results are represented as the mean and the SD.