![Figure 4. BTN1 overexpression or deletion has opposing effects on Golgi morphology. (A) Deletion of BTN1 enhances Golgi clustering in cells expressing GFP-Sed5. WT, btn1Δ, and WT cells overexpressing BTN1 from a multicopy plasmid (BTN1) and all expressing GFP-Sed5 from a single-copy plasmid are shown. Note the large puncta in btn1Δ cells. (B) Deletion of BTN1 enhances Golgi clustering in cells expressing GFP-Sed5317A, whereas BTN1 overexpression enhances fragmentation (same as in A, except all strains express GFP-Sed5317A). Note the large puncta in WT and btn1Δ cells. (C) Golgi clustering is reduced in btn1Δ cells expressing GFP-Sed5317D (same as in A, except all strains express GFP-Sed5317D). (D) Deletion of BTN1 enhances Golgi clustering in cells expressing GFP-Sed5 from its genomic locus (GFP-Sed5 integrated [int.]). WT, btn1Δ, and WT cells overexpressing BTN1 from a multicopy plasmid (BTN1) and all expressing GFP-SED5 from its genomic locus are shown. Note the larger puncta in btn1Δ cells. (E) BTN1 overexpression reduces Golgi clustering in cells expressing GFP-Sed5317A from its genomic locus (same as in D, except all cells express GFP-SED5317A). Note the reduction in puncta size in cells overproducing Btn1. (F) Deletion of BTN1 enhances Golgi clustering in cells expressing GFP-Sed5317D from its genomic locus (same as in D, except all cells express GFP-SED5317D). Note the enhancement in puncta size in cells lacking BTN1. Bars, 1 µm.](https://cdn.rupress.org/rup/content_public/journal/jcb/195/2/10.1083_jcb.201102115/7/jcb_201102115_rgb_fig4.jpeg?Expires=1773560084&Signature=EJbXjWIejxHoI8C9eMur9vDJcy05jgByko3ci8nQXcRRWNvtnH1OyUFygC-CJUPNovzT3fqWC5FkHB0-uC3gna6zKBXtM8zU6K9u9Nr5w121QAqPatIXQPhWmwLNm~v6mzanfG-Msje~vOZBUJw7krRiqt0DiyAgN2fb6Jes94xQtyLvMDFjdLUrpEjG9RFSPXnKOFCSV4-fmtxrsNoFPbTIN2MatyhPOm3el1LZZzWecGbkR4NaGBOP3QSSffwFhD1K0jSocHgNDw8SRSSAU9sBJTR5BKP52a8kKlVH7yoZUYj3Of8uD9QagG6Ex9Us9E9v5nCbcVZkVpYQJTKktQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
BTN1 overexpression or deletion has opposing effects on Golgi morphology. (A) Deletion of BTN1 enhances Golgi clustering in cells expressing GFP-Sed5. WT, btn1Δ, and WT cells overexpressing BTN1 from a multicopy plasmid (BTN1) and all expressing GFP-Sed5 from a single-copy plasmid are shown. Note the large puncta in btn1Δ cells. (B) Deletion of BTN1 enhances Golgi clustering in cells expressing GFP-Sed5317A, whereas BTN1 overexpression enhances fragmentation (same as in A, except all strains express GFP-Sed5317A). Note the large puncta in WT and btn1Δ cells. (C) Golgi clustering is reduced in btn1Δ cells expressing GFP-Sed5317D (same as in A, except all strains express GFP-Sed5317D). (D) Deletion of BTN1 enhances Golgi clustering in cells expressing GFP-Sed5 from its genomic locus (GFP-Sed5 integrated [int.]). WT, btn1Δ, and WT cells overexpressing BTN1 from a multicopy plasmid (BTN1) and all expressing GFP-SED5 from its genomic locus are shown. Note the larger puncta in btn1Δ cells. (E) BTN1 overexpression reduces Golgi clustering in cells expressing GFP-Sed5317A from its genomic locus (same as in D, except all cells express GFP-SED5317A). Note the reduction in puncta size in cells overproducing Btn1. (F) Deletion of BTN1 enhances Golgi clustering in cells expressing GFP-Sed5317D from its genomic locus (same as in D, except all cells express GFP-SED5317D). Note the enhancement in puncta size in cells lacking BTN1. Bars, 1 µm.
