Figure 2.
Figure 2. Tmods and γcyto-actin exhibit two distinct extrasarcomeric localizations at the M line and flanking the Z line, and deletion of Tmod1 depletes γcyto-actin from the M line. (A–C) Longitudinal cryosections of 1-mo-old TA muscle fibers from Tmod1+/+Tg+ and Tmod1−/−Tg+ mice immunostained for γcyto-actin, F-actin, and either Tmod1 (A), Tmod3 (B), or β2-spectrin (C). (D and E) Quantitation of the ratio of M/Z (D) and Z/P (E) fluorescence of γcyto-actin, β2-spectrin, and Tmods. Each bar reflects n = 91–99 myofibrils/genotype measured from three mice (six muscles)/genotype for γcyto-actin, β2-spectrin, and Tmod3 or n = 42–83 myofibrils/genotype measured from two mice (four muscles)/genotype for Tmod1 and Tmod4. *, P < 0.001. M, M line; P, thin filament pointed ends; Z, Z line. Data are presented as mean ± SD. Bars, 1 µm.

Tmods and γcyto-actin exhibit two distinct extrasarcomeric localizations at the M line and flanking the Z line, and deletion of Tmod1 depletes γcyto-actin from the M line. (A–C) Longitudinal cryosections of 1-mo-old TA muscle fibers from Tmod1+/+Tg+ and Tmod1−/−Tg+ mice immunostained for γcyto-actin, F-actin, and either Tmod1 (A), Tmod3 (B), or β2-spectrin (C). (D and E) Quantitation of the ratio of M/Z (D) and Z/P (E) fluorescence of γcyto-actin, β2-spectrin, and Tmods. Each bar reflects n = 91–99 myofibrils/genotype measured from three mice (six muscles)/genotype for γcyto-actin, β2-spectrin, and Tmod3 or n = 42–83 myofibrils/genotype measured from two mice (four muscles)/genotype for Tmod1 and Tmod4. *, P < 0.001. M, M line; P, thin filament pointed ends; Z, Z line. Data are presented as mean ± SD. Bars, 1 µm.

This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal