The α-synuclein chimeric probe colocalizes specifically with endocytic and post-Golgi markers. (A–C) Localization of the α-synuclein chimera–GFP (α-syn chim) with the indicated mCherry-tagged marker. Representative images for mCherry-Bos1p (A) and mCherry-Snc1p (B) are shown. Quantifications are depicted in C. Cells were grown overnight under repressing conditions and then transferred to induction medium for 2.5 (A–C) or 4 h (C). Means and SDs of at least three independent experiments are shown. Bar, 2 µm.