Figure 8. Tip cell abnormalities in nrp2+/−vegfr3+/− mice are similar to those obtained after anti-Nrp2B treatment. (A and B) X-gal staining of P5 hearts from mice of the indicated genotypes. Note regular branching of lymphatics in the vegfr3+/− heart (A) but enlarged, poorly branched vessels devoid of sprouting tips in the double-heterozygote heart (B). (C–F) Lymphatic sprouting defects in the small intestine at P4. LYVE-1–stained sections show presence of lymphatics in almost all villi of wild-type mice (C) but virtually no sprouts in most sections of the double-heterozygous mice (D). Only some sections show a portion of the villi-containing lymphatics (E), which are of normal length compared with wild type (C). (F) Quantification of the percentage of villi-containing lymphatics. *, P < 0.01. Error bars indicate SEM. Bars: (A and B) 210 µm; (C–E) 200 µm.
Figure 8.

Tip cell abnormalities in nrp2+/−vegfr3+/− mice are similar to those obtained after anti-Nrp2B treatment. (A and B) X-gal staining of P5 hearts from mice of the indicated genotypes. Note regular branching of lymphatics in the vegfr3+/− heart (A) but enlarged, poorly branched vessels devoid of sprouting tips in the double-heterozygote heart (B). (C–F) Lymphatic sprouting defects in the small intestine at P4. LYVE-1–stained sections show presence of lymphatics in almost all villi of wild-type mice (C) but virtually no sprouts in most sections of the double-heterozygous mice (D). Only some sections show a portion of the villi-containing lymphatics (E), which are of normal length compared with wild type (C). (F) Quantification of the percentage of villi-containing lymphatics. *, P < 0.01. Error bars indicate SEM. Bars: (A and B) 210 µm; (C–E) 200 µm.

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