UBC7 and UFD4 are not required for increased degradation at 38°C and after exposure to paraquat or cadmium. (A) WT and Δubc7 strains were labeled with ³⁵S-Met for 5 min at 30°C, and rates of degradation at 38°C and upon exposure to paraquat or cadmium were measured as in Fig. 6 A. (B) WT and Δufd4 strains were labeled with ³⁵S-Met for 5 min at 30°C, and rates of degradation at 38°C and upon exposure to paraquat or cadmium were measured as in Fig. 6 A.