Figure 4.

Shift to 38°C stimulates the degradation of recently synthesized proteins in WT but not in Δrpn10 and Δubc4Δubc5 strains. Cells were labeled with 35S-Met for 5 min at 30°C and shifted to 38°C or maintained at 30°C. Rates of degradation were measured as in Fig. 1 A.

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