Figure 6. Phosphorylation of MyoD by p38-γ enhances its occupancy on the Myogenin promoter. (A) Endogenous MyoD ChIP assay on C2C12 myoblasts expressing p38-γ, MKK6EE and p38-γ, or empty vector after 24 h of differentiation. 10T1/2 fibroblast extracts were used as control. (B) MyoD ChIP assay from 10T1/2 fibroblasts expressing MKK6EE and p38-γ, or empty vector after 24 h of differentiation. Wild-type (wt) and mutant (mut) MyoD S199A/S200A were expressed as indicated. PCR was performed using primers spanning the Igh enhancer as a control. Fold changes for each condition relative to empty vector (C2C12) or wild-type MyoD (10T1/2) are shown below representative gels.
Figure 6.

Phosphorylation of MyoD by p38-γ enhances its occupancy on the Myogenin promoter. (A) Endogenous MyoD ChIP assay on C2C12 myoblasts expressing p38-γ, MKK6EE and p38-γ, or empty vector after 24 h of differentiation. 10T1/2 fibroblast extracts were used as control. (B) MyoD ChIP assay from 10T1/2 fibroblasts expressing MKK6EE and p38-γ, or empty vector after 24 h of differentiation. Wild-type (wt) and mutant (mut) MyoD S199A/S200A were expressed as indicated. PCR was performed using primers spanning the Igh enhancer as a control. Fold changes for each condition relative to empty vector (C2C12) or wild-type MyoD (10T1/2) are shown below representative gels.

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