Figure 2. Precocious up-regulation of Myogenin in p38-γ–deficient muscle. (A–F) Immunofluorescent staining for Myogenin (B and E) from wild-type and p38-γ−/− TA muscle. Nuclei were counterstained with DAPI (A and D). (C and F) Myogenin-DAPI merged pictures. Representative pictures are shown (n = 9). (G–P) Immunofluorescent staining for Pax7 (H and L) and Myogenin (I and M) in single EDL fibers from wild-type mice transfected with siRNA against p38-γ or control scrambled siRNA immediately after their isolation. Nuclei were counterstained with DAPI (G and K). (J and N) Pax7-Myogenin-DAPI merged pictures. Representative pictures are shown. The percentage of Myogenin-expressing and Pax7-expressing myogenic precursor cells was determined 72 h after transfection (O and P). Error bars represent ±SEM for n = 28. Asterisks denote significance (P < 0.001). Bars, 25 µm.
Figure 2.

Precocious up-regulation of Myogenin in p38-γ–deficient muscle. (A–F) Immunofluorescent staining for Myogenin (B and E) from wild-type and p38-γ−/− TA muscle. Nuclei were counterstained with DAPI (A and D). (C and F) Myogenin-DAPI merged pictures. Representative pictures are shown (n = 9). (G–P) Immunofluorescent staining for Pax7 (H and L) and Myogenin (I and M) in single EDL fibers from wild-type mice transfected with siRNA against p38-γ or control scrambled siRNA immediately after their isolation. Nuclei were counterstained with DAPI (G and K). (J and N) Pax7-Myogenin-DAPI merged pictures. Representative pictures are shown. The percentage of Myogenin-expressing and Pax7-expressing myogenic precursor cells was determined 72 h after transfection (O and P). Error bars represent ±SEM for n = 28. Asterisks denote significance (P < 0.001). Bars, 25 µm.

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