Figure 6. Sorting of GPI-APs to the ERES is dependent on p24 family. (A and B) CHO-K1 cells stably expressing Venus-CD59 were transfected with control siRNA (A) or siRNA against p23 (B). After 72 h, the cells were treated with PI-PLC, followed by fixation, permeabilization, and immunostaining with anti-p23. Arrows indicate efficiently silenced cells. (C and D) CHO-K1 cells stably expressing Venus-CD59 were transfected with control RNA (C) or siRNA against p23 (D). After 72 h, the cells were treated with PI-PLC, incubated at 10°C for 1 h, fixed with 4% paraformaldehyde, and stained with anti-Sec13 to observe the ERES. In the merged images, Sec13 and Venus-CD59 were shown in red and green, respectively. Enlarged views of the boxed regions are shown below. Bars, 10 µm.
Figure 6.

Sorting of GPI-APs to the ERES is dependent on p24 family. (A and B) CHO-K1 cells stably expressing Venus-CD59 were transfected with control siRNA (A) or siRNA against p23 (B). After 72 h, the cells were treated with PI-PLC, followed by fixation, permeabilization, and immunostaining with anti-p23. Arrows indicate efficiently silenced cells. (C and D) CHO-K1 cells stably expressing Venus-CD59 were transfected with control RNA (C) or siRNA against p23 (D). After 72 h, the cells were treated with PI-PLC, incubated at 10°C for 1 h, fixed with 4% paraformaldehyde, and stained with anti-Sec13 to observe the ERES. In the merged images, Sec13 and Venus-CD59 were shown in red and green, respectively. Enlarged views of the boxed regions are shown below. Bars, 10 µm.

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