Figure 9. MPTAs in Smc1β−/− oocytes. (A and B) IF images showing a variety of aberrations in pachytene oocytes. (A) Telomere bridges visualized by RAP1 staining (green) between two or three SCs (SYCP3, red). (B) Gaps (arrow) between the telomeric signal (RAP1, green) and the SC (SYCP3, red; left inset), and split telomeres at one end and no telomere signal on the other end of a chromosome (right inset). (C and D) Quantification of telomere length of zygotene oocytes (n = 10 each). (C) Intensity plot of the telomere signal gained by G-strand telo-FISH. (D) Whisker box plot of WT and Smc1β−/− (KO) cells showing the median telomere length (−) and the maximum length range (+). (E) WT oocyte control staining. Insets indicate individual chromosome magnifications of the indicated boxed areas. Bars: (whole nucleus images) 10 µm; (insets) 2 µm.
Figure 9.

MPTAs in Smc1β−/− oocytes. (A and B) IF images showing a variety of aberrations in pachytene oocytes. (A) Telomere bridges visualized by RAP1 staining (green) between two or three SCs (SYCP3, red). (B) Gaps (arrow) between the telomeric signal (RAP1, green) and the SC (SYCP3, red; left inset), and split telomeres at one end and no telomere signal on the other end of a chromosome (right inset). (C and D) Quantification of telomere length of zygotene oocytes (n = 10 each). (C) Intensity plot of the telomere signal gained by G-strand telo-FISH. (D) Whisker box plot of WT and Smc1β−/− (KO) cells showing the median telomere length (−) and the maximum length range (+). (E) WT oocyte control staining. Insets indicate individual chromosome magnifications of the indicated boxed areas. Bars: (whole nucleus images) 10 µm; (insets) 2 µm.

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