Telomere aberrations and SUN1 association. (A) Correlation of the mean telomere length as assessed by Q-FISH, i.e., telomere signal intensity with presence or absence of SUN1. In WT, all telomeres are associated with SUN1. (B) Distribution of telomere length and association with SUN1 (SUN1pos and SUN1neg) for Smc1β^−/− pachytene spermatocytes; the WT distribution of telomere length is shown for comparison. (C–E) Telo-FISH combined with IF staining for SYCP3 and SUN1 in WT (C) and Smc1β^−/− spermatocytes (D and E). (E) Individual aberrant telomere structures from D as indicated (arrows) or from similar images. Error bars indicate SD. Bars, 10 µm.