Figure 5. Structural aberrations at chromosome ends in Smc1β−/− spermatocytes. Analysis of G-strands by telo-FISH (green) and costaining for the SC (red, SYCP3) and DAPI of spermatocyte spreads. (A–D) Graphs show mean numbers of aberrations in WT and Smc1β−/− cells at zygotene and pachytene. (A) Total number of telomeres per cell. (B) Number of SC-less telomeres per cell. (C) Number of telo-less SCs per cell. (D) Numbers of telomere stretches per cell. (E) Number of telomere bridges per cell. Images show examples of the aberrations in Smc1β−/− pachytene cells. P < 0.0001. (F) Smc1β+/− pachytene cell. (G) Smc1β−/− cells. White arrows, SC-less telomeres; gray arrows, telo-less SCs; turquoise arrows, stretches; yellow arrow, unpaired telomeres. Boxed areas show apparent bridges and stretches, which are shown in higher magnification in insets. Error bars indicate SD. Bars, 10 µm (except in insets).
Figure 5.

Structural aberrations at chromosome ends in Smc1β−/− spermatocytes. Analysis of G-strands by telo-FISH (green) and costaining for the SC (red, SYCP3) and DAPI of spermatocyte spreads. (A–D) Graphs show mean numbers of aberrations in WT and Smc1β−/− cells at zygotene and pachytene. (A) Total number of telomeres per cell. (B) Number of SC-less telomeres per cell. (C) Number of telo-less SCs per cell. (D) Numbers of telomere stretches per cell. (E) Number of telomere bridges per cell. Images show examples of the aberrations in Smc1β−/− pachytene cells. P < 0.0001. (F) Smc1β+/− pachytene cell. (G) Smc1β−/− cells. White arrows, SC-less telomeres; gray arrows, telo-less SCs; turquoise arrows, stretches; yellow arrow, unpaired telomeres. Boxed areas show apparent bridges and stretches, which are shown in higher magnification in insets. Error bars indicate SD. Bars, 10 µm (except in insets).

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