Figure 4.
![Figure 4. Telomere length analysis. (A) Assessment of telomere length in WT or Smc1β−/− spermatocytes. Southern blotting and ethidium bromide (EtBr)–stained gel for loading control of Smc1β+/− testis DNA (Het) and Smc1β−/− testis DNA (KO). m, DIG-labeled marker; M, pulsed field gel electrophoresis low-range marker. Bars indicate the length of the telomere signal smear. (B) Quantitative telo-FISH of zygotene or pachytene spermatocyte spreads such as those shown in Figs. 6 and 7 using ImageJ software. (C) Box and whiskers plot to visualize median lengths (−) and maximum range (+). The intervals containing the median 50% of telomere intensity values are boxed (7.8–22.5 fluorescence telomere intensity units [F-TIU]).](https://cdn.rupress.org/rup/content_public/journal/jcb/187/2/10.1083_jcb.200808016/6/jcb_200808016_gs_fig4.jpeg?Expires=1767708744&Signature=JWszSqmIkSiV4-YekUgYQw~LRoUTS55nvaEDNdeHuWZsuT9LIdfubEYYzWd2fAnQ3ncJ8sXmmTSey7pdWY0OrDeJ-R99iAX~n30Uuvu940lZLiM-71LwJKw5S-YFkeQJfIlO8C0FAK6RsCAW5v-sBLe8pg6KjlH-hgx9m8XCq39vyypVxTMJuqEYsz6Q1vWM1MqMD7xbIJE89cBHS01tICvLBTGv-7tFQl1VkOuABNz9k8Xo8J07d~Iapk9~mf1Ufk05muzDTGmU3j0kLFXSR8jLZbbVj2lexqrqbavAlbSIu9lw29ZL43iLMF9UaOXReVtYui0roCg1kEh4NAC-1Q__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Telomere length analysis. (A) Assessment of telomere length in WT or Smc1β−/− spermatocytes. Southern blotting and ethidium bromide (EtBr)–stained gel for loading control of Smc1β+/− testis DNA (Het) and Smc1β−/− testis DNA (KO). m, DIG-labeled marker; M, pulsed field gel electrophoresis low-range marker. Bars indicate the length of the telomere signal smear. (B) Quantitative telo-FISH of zygotene or pachytene spermatocyte spreads such as those shown in Figs. 6 and 7 using ImageJ software. (C) Box and whiskers plot to visualize median lengths (−) and maximum range (+). The intervals containing the median 50% of telomere intensity values are boxed (7.8–22.5 fluorescence telomere intensity units [F-TIU]).
