The CPC and CENP-E exhibit distinct central spindle transfer mechanisms during anaphase. (A–F) CENP-E transfers to the central spindle at anaphase even when the CPC remains stuck on the chromatin. CENP-E (green; panels 3 and 7) and α-tubulin or TrAP-INCENP (red; panels 2 and 6) plus DAPI for DNA (blue; panels 4 and 8) are shown for INCENP^ON cells (A), INCENP^OFF cells (B), or INCENP^OFF cells expressing TrAP-INCENP^WT (C), TrAP-INCENP^W766G (D), TrAP-INCENP^F802A (E), or TrAP-INCENP^WT in the presence of ZM447439 (F). CENP-E localizes to the outer kinetochore in all cells (insets) and transfers to the central spindle at anaphase, even in cells expressing TrAP-INCENP^W766G or TrAP-INCENP^F802A in which the CPC remains trapped on the chromatin. Insets show magnified views of boxed regions. Bars, 5 µm. (G) The level of H3S10ph is reduced significantly in the presence of ZM447439 as a result of aurora B inhibition. Levels of aurora B and PP1 remained the same. α-Tubulin was used as a loading control.