Kinetochore assembly is normal in cells lacking a functional CPC. (A–E) Kinetochore proteins CENP-A (A), CENP-H (B), CENP-O (C), CENP-T (D), and Hec1 (E) localize normally during metaphase and anaphase in INCENP^ON cells (panels 1–8) and INCENP^OFF cells (panels 9–16). CENP proteins are shown in green, α-tubulin or CENP-H–GFP in red, and DAPI (DNA) in blue. Insets show magnified views of boxed regions. Bars, 5 µm.