Immunogold localization of LC1 within oda4-s7 oda11 axonemes. Electron micrographs of cross sections through an oda4-s7 oda11 axoneme (top) and individual outer doublets (middle) that had been incubated with antibody against LC1 and a secondary antibody conjugated to 5 nm gold. (bottom) A diagram illustrating the outer arm from this strain (yellow) and the location of individual gold particles (marked by x) is shown. The gold particles are clustered near the A-tubule of the doublet to which the outer arm is permanently attached. Arrows indicate two individual gold particles. Bar, 25 nm.