Figure 4.
Figure 4. Motile behavior of transformed strains. (a) The beat frequency power spectra for the cwd arg7-8 parental strain and transformants expressing myc-tagged LC1 and the M182A, M182P, D185G, D185P, R189A, and R196A mutant forms are shown. Strains incorporating the myc tag alone or the M182A, M182P, and D185G mutant forms have a beat frequency of ∼40–45, Hz which is similar to the parental strain. Both R189A and R196A mutants had beat frequencies reduced somewhat to 35–40 Hz, and only D185P exhibited a more serious reduction to ∼32 Hz. (b) The paths taken by individual cells expressing myc-tagged LC1 and the R196D and R189A mutant forms were tracked using MetaMorph. Most mutant strains moved in a helical path with occasional alterations in swimming direction as do wild-type cells. However, several R189A transformants swam very slowly and exhibited highly convoluted tracks as they continually altered direction.

Motile behavior of transformed strains. (a) The beat frequency power spectra for the cwd arg7-8 parental strain and transformants expressing myc-tagged LC1 and the M182A, M182P, D185G, D185P, R189A, and R196A mutant forms are shown. Strains incorporating the myc tag alone or the M182A, M182P, and D185G mutant forms have a beat frequency of ∼40–45, Hz which is similar to the parental strain. Both R189A and R196A mutants had beat frequencies reduced somewhat to 35–40 Hz, and only D185P exhibited a more serious reduction to ∼32 Hz. (b) The paths taken by individual cells expressing myc-tagged LC1 and the R196D and R189A mutant forms were tracked using MetaMorph. Most mutant strains moved in a helical path with occasional alterations in swimming direction as do wild-type cells. However, several R189A transformants swam very slowly and exhibited highly convoluted tracks as they continually altered direction.

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