Figure 3. Myc-tagged LC1 copurifies with... | Rockefeller University Press

Figure 3.

$Figure 3. Myc-tagged LC1 copurifies with the outer dynein arm. (a) Immunoblots of flagellar fractions from strains expressing either myc-tagged LC1 or the D185G mutant form are shown. Addition of the myc tag alone (top) and most mutant forms did not alter either the detergent or 0.6 M NaCl extraction properties of LC1. In contrast, the D185G and M182G (not depicted) versions of LC1 exhibited an enhanced tendency to dissociate after detergent treatment of flagella (bottom). (b) Immunoblot of dynein from a strain expressing the M182G myc-tagged form of LC1 fractionated in a 5–20% sucrose density gradient; the bottom of the gradient is at left. The altered version of LC1 precisely comigrates with the native protein. Numbers in parentheses are given in kD.$

Myc-tagged LC1 copurifies with the outer dynein arm. (a) Immunoblots of flagellar fractions from strains expressing either myc-tagged LC1 or the D185G mutant form are shown. Addition of the myc tag alone (top) and most mutant forms did not alter either the detergent or 0.6 M NaCl extraction properties of LC1. In contrast, the D185G and M182G (not depicted) versions of LC1 exhibited an enhanced tendency to dissociate after detergent treatment of flagella (bottom). (b) Immunoblot of dynein from a strain expressing the M182G myc-tagged form of LC1 fractionated in a 5–20% sucrose density gradient; the bottom of the gradient is at left. The altered version of LC1 precisely comigrates with the native protein. Numbers in parentheses are given in kD.