Figure 5. Dominant-negative erasin... | Rockefeller University Press

Figure 5.

$Figure 5. Dominant-negative erasin fragments interfere with CD3δ degradation. (A) HEK293-CD3δ cells were either mock transfected (control) or transfected with erasin deletion constructs encoding portions of the erasin polypeptide from amino acids 1–310, 414–508, or 1–168. Protein turnover was analyzed similar to Fig 4. Both a long and short exposure of the CD3δ signal is shown. Please note that the erasin 414–508 runs aberrantly on SDS-PAGE. (B) Graphs of CD3δ turnover determined from three separate experiments showing the mean and SD (error bars). (C) Immunoblots showing ubiquilin and erasin distribution in fractions of HEK293-CD3δ cell homogenates separated on 0–25% iodixanol gradients.$

Dominant-negative erasin fragments interfere with CD3δ degradation. (A) HEK293-CD3δ cells were either mock transfected (control) or transfected with erasin deletion constructs encoding portions of the erasin polypeptide from amino acids 1–310, 414–508, or 1–168. Protein turnover was analyzed similar to Fig 4. Both a long and short exposure of the CD3δ signal is shown. Please note that the erasin 414–508 runs aberrantly on SDS-PAGE. (B) Graphs of CD3δ turnover determined from three separate experiments showing the mean and SD (error bars). (C) Immunoblots showing ubiquilin and erasin distribution in fractions of HEK293-CD3δ cell homogenates separated on 0–25% iodixanol gradients.