Pex3-1p fails to recruit Inp1p-GFP to peroxisomes. (A) WT and pex3-1 cells expressing Inp1p-GFP at endogenous levels were mated with pex3Δ cells expressing HcRed-PTS1 and imaged after 2 h. (B) The cytosolic domain of Pex3p and Pex3-1p was redirected to mitochondria by fusion to Tom70p in pex3Δ cells expressing Inp1p-GFP at endogenous levels. Mito-Pex3-mRFP and mito-Pex3-1-mRFP expression was induced on galactose medium for times indicated. Signals of mito-Pex3p and mito-Pex3-1p are directly comparable; Inp1p-GFP signals are more enhanced in pex3-1 cells. The strains were grown on selective medium and examined by epifluorescence and phase contrast. Bar, 5 µm.