Figure 2. Inp1p localization in peroxisome-deficient cells. A plasmid that expresses Inp1p-GFP under the control of its endogenous promoter was transformed into WT (A), pex19Δ (A–C), and pex3Δ (A) cells expressing HcRed-PTS1 (A), Sec66p-HcRed (B), and Pex3p-mRFP (C). The strains were grown on selective medium and examined by epifluorescence and phase contrast. For A and C, multiple epifluorescence images were acquired in the z axis and flattened into a single image. For B, a single focal plane was taken. Bar, 5 µm.
Figure 2.

Inp1p localization in peroxisome-deficient cells. A plasmid that expresses Inp1p-GFP under the control of its endogenous promoter was transformed into WT (A), pex19Δ (A–C), and pex3Δ (A) cells expressing HcRed-PTS1 (A), Sec66p-HcRed (B), and Pex3p-mRFP (C). The strains were grown on selective medium and examined by epifluorescence and phase contrast. For A and C, multiple epifluorescence images were acquired in the z axis and flattened into a single image. For B, a single focal plane was taken. Bar, 5 µm.

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