Figure 1. Isolation of a new class of pex3 mutants. (A) pex3Δ cells expressing the lumenal peroxisomal marker GFP-PTS1 were transformed with plasmids containing a WT PEX3 allele or a representative member of each pex3 mutant class. inp1Δ cells are included for comparison. (B) Quantitative description of peroxisome distribution in WT, pex3Δ, inp1Δ, inp2Δ, and the class III mutants pex3-1 and pex3-2. Overnight cultures were diluted and grown for 6 h in selective glucose medium, examined by epifluorescence and phase contrast, and scored for peroxisome distribution. More than 100 budding cells were analyzed for each strain. Three independent experiments were performed. Error bars represent SEM. (C) pex3-1 cells expressing GFP-PTS1 were spotted on an agarose pad and peroxisome distribution was followed with time. A and C show merged brightfield (blue) and fluorescent images (green). Bar, 5 µm.
Figure 1.

Isolation of a new class of pex3 mutants. (A) pex3Δ cells expressing the lumenal peroxisomal marker GFP-PTS1 were transformed with plasmids containing a WT PEX3 allele or a representative member of each pex3 mutant class. inp1Δ cells are included for comparison. (B) Quantitative description of peroxisome distribution in WT, pex3Δ, inp1Δ, inp2Δ, and the class III mutants pex3-1 and pex3-2. Overnight cultures were diluted and grown for 6 h in selective glucose medium, examined by epifluorescence and phase contrast, and scored for peroxisome distribution. More than 100 budding cells were analyzed for each strain. Three independent experiments were performed. Error bars represent SEM. (C) pex3-1 cells expressing GFP-PTS1 were spotted on an agarose pad and peroxisome distribution was followed with time. A and C show merged brightfield (blue) and fluorescent images (green). Bar, 5 µm.

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