SA1 and TIN2, but not SA2, are required for sister chromatid cohesion along arms. (A–D) Chromosome spread analysis of siRNA-treated HeLaI.2.11 cells stained with antibodies to the condensin subunit Smc2. Histograms (based on ∼110 measurements from three spreads [see Table S2]) showing the distance between arms are on the right. Average distance (Avg) with SEs is indicated. (E) Graphical representation of the frequency of each type of chromosome morphology. The classification was assigned when five or more chromosomes in a spread displayed the indicated morphology. Bar graphs represent the average values from three independent experiments with SDs (see Table I). (F–H) Chromosome spread analysis from double siRNA–treated cells. 150–200 spreads were scored (see Table S3). The percentage indicates the frequency of the indicated morphology. Bars, 5 μm.